As a result of Jantjies' injury, Dan Kriel (centre) - twin brother of Springbok midfielder Jesse Kriel - will lead the Emirates Lions at Emirates Airline Park, with Gianni Lombard taking over at flyhalf.
The match on Saturday kicks off at 16h30 and will be broadcast live on SuperSport.
In the only other change to the backline this weekend, Wandisile Simelane comes in to rekindle his partnership with Kriel.
Amongst the forwards, MJ Pelser gets the nod at flank in the only change to the pack.
Emirates Lions coach Ivan van Rooyen has called up a bunch of new players to the bench though - PJ Botha, Jannie du Plessis, Nathan McBeth, Marnus Schoeman, Stean Pienaar and Tiaan Swanepoel.
Emirates Lions: 15 EW Viljoen, 14 Jamba Ulengo, 13 Wandisile Simelane, 12 Dan Kriel (captain), 11 Rabz Maxwane, 10 Gianni Lombard, 9 Morné van den Berg, 8 Len Massyn, 7 Vincent Tshituka, 6 MJ Pelser, 5 Marvin Orie, 4 Ruben Schoeman, 3 Carlu Sadie, 2 Jaco Visagie, 1 Sti Sithole. Replacements: 16 PJ Botha, 17 Nathan McBeth, 18 Jannie du Plessis, 19 Wilhelm van der Sluys, 20 Marnus Schoeman, 21 Ross Cronje, 22 Stean Pienaar, 23 Tiaan Swanepoel.
Match information - Emirates Lions vs Toyota Cheetahs
Date: Saturday, 24 October
Venue: Emirates Airline Park, Johannesburg
Referee: Rasta Rasivhenge
Assistant Referees: Aimee Barrett-Theron, Griffin Colby
Television Match Official: Willie Vos
TV: SuperSport Grandstand and Rugby