The Kings remain at the foot of the Conference with just a single win this season, but they impressed at the Sportsground and scored two tries through Erich Cronje and Scott van Breda.
Connacht, who arrived with four wins from six league games, started impressively, with Ireland international centre Aki powering over in the 12th minute. However the PE side, filled with confidence from securing their first ever Guinness PRO14 away win at Ospreys last time out, hit back as centre Erich Cronje wrestled his way over four minutes later.
But it was Connacht who showed greater forward power and they moved back ahead mid-way through the first half thanks to tries from prop Finlay Bealham and wing John Porch. The Kings hit back on the stroke of half-time through full-back Scott van Breda, who only joined on loan from Worcester Warriors this week.
Connacht strengthened their advantage early in the second half, as Conor Fitzgerald and Bundee Aki combined to release Porch, who sprinted in for his second try in the corner. With two predatory tries, the wing made the difference as the Irish province once again showed how dangerous they will be this season.
Connacht 24 - Tries: Bundee Aki, Finlay Bealham, John Porch (2); Conversions: Conor Fitzgerald (2).
Isuzu Southern Kings 12 - Tries: Erich Cronje, Scott van Breda; Conversion: Van Breda.